Regulation of papillomavirus transcription and replication; insights for the design of extrachromosomal vectors
Alison A. McBride
Received: 16 October 1998; accepted: 20 October 1998
The papillomaviruses infect and replicate in the stratified layers of skin and mucosa and give rise to benign lesions called warts or papillomas. The virus infects basal epithelial cells and within these persistently infected cells the viral genome is maintained at low levels as extrachromosomally replicating viral DNA. The genomes of papillomaviruses can also be stably maintained as high copy number extrachromosomal elements in cell lines and within these cells the viral genomes replicate in synchrony with cellular DNA. The E1 and E2 viral proteins regulate viral transcription, initiation of replication and long term episomal maintenance of viral genomes. This review will describe the functions of the E1 and E2 proteins and discuss how these functions can be exploited in the design of extrachromosomal replicating vectors for gene therapy.
Certain DNA viruses, such as papillomavirus or Epstein-Barr virus, are able to maintain their genomes as stable extrachromosomal elements in the nuclei of infected cells. The papillomaviruses are small DNA viruses that infect basal epithelial cells and replicate in terminally differentiating keratinocytes. These viruses have been isolated from a wide range of vertebrates and they exhibit both host species and tissue specificity. Viral DNA replication has been studied mostly in bovine papillomavirus type 1 (BPV-1) and the human papillomaviruses (HPV), HPV-1, -11, -16 and -18 and -31.
The viral E1 and E2 proteins are important for initiation of viral DNA replication and for regulation of viral transcription. The E1 protein is the primary viral replication initiator protein (Ustav and Stenlund, 1991a; Mohr et al., 1990; Yang et al., 1991) and E1 also functions as a transcriptional repressor (Sandler et al., 1993; Le Moal et al., 1994); the viral E2 protein(s) are transcriptional regulatory proteins that regulate the expression of the other viral gene products and, in addition, play an important role in DNA replication. The E2 transactivator protein is also required for long-term episomal maintenance of viral genomes within replicating cells (Piirsoo et al., 1996). Papillomavirus genomes and the E2-TA protein interact with mitotic chromosomes in dividing cells and this association is likely to be important for genome segregation (Skiadopoulos and McBride, 1998; Lehman and Botchan, 1998).
Papillomaviruses infect and replicate in stratified epithelium and give rise to benign lesions called warts or papillomas. Papillomaviruses infect the lower basal layer of cells of a stratified epithelium (Figure 1). The a6b4 integrin protein, expressed exclusively in this cell layer, acts as a receptor for the virus (Evander et al., 1997). Damage to the superficial layers of the epithelium is probably necessary to allow access of virus to the basal layer. Within basal cells the viral genome is amplified to a low copy number and maintained as an extrachromosomally replicating circle of double stranded DNA (Figure 2). DNA replication in these cells probably requires the viral E1 and E2 replication proteins. The viral E5 protein is also expressed in basal cells. E5
stimulates the activity of growth factor receptors expressed by the cell and induces cellular proliferation (reviewed in Howley, 1995). Enhanced proliferation of basal cells may be important to increase the population of infected cells and to provide a suitable environment for establishment of a productive viral lesion. As basal cells differentiate and migrate up to the stratum spinosum, expression of the E2 proteins is greatly increased and vegetative DNA replication begins (Burnett et al., 1990; Howley, 1995). The cells in this layer do not normally divide nor express cellular proteins necessary for DNA replication. Therefore, the viral E7 protein is required to induce the differentiated keratinocytes to enter S-phase and synthesize cellular replication proteins by binding to and inactivating the cellular retinoblastoma protein, Rb (reviewed in Jones and Munger, 1996). However, the conflicting signals of cell cycle progression and differentiation induce the p53 protein, which in turn signals cells to undergo apoptosis or growth arrest. The viral E6 protein can inactivate this function of p53 by targeting it for degradation by the ubiquitin-proteasome pathway (reviewed in Kubbutat and Vousden, 1996). The viral E4 protein is also abundant in the more differentiated layers of a papilloma. It has been hypothesized that E4 may function as a nuclear structural protein, an RNA splicing and transport factor, or in release of viral particles from the papilloma (reviewed in Howley, 1995). In the upper differentiated layers of the papilloma, the viral capsid proteins L1 and L2 are synthesized and virions are assembled (reviewed in Howley, 1995).
Three modes of DNA replication take place in the papillomavirus life cycle: initial DNA amplification, maintenance replication and vegetative replication. After initial uptake of the virus, the virion particle is uncoated and the genome transported to the nucleus of the basal cell where it is presumed to be amplified to a low copy number (Zhou et al., 1995). Presumably, a low level of the E1 and E2 proteins must be expressed early after infection since there is no evidence that they are in the viral particle. Most experimental studies have examined transient DNA replication in cultured cells, a system that is probably most analogous to this initial amplification stage and which requires the E1 and E2 proteins and the viral replication origin (Ustav and Stenlund, 1991a; Ustav and Stenlund, 1991a).
Stable episomal maintenance is the second stage of papillomavirus DNA replication. In a papilloma, the infected basal cells proliferate and maintain low levels of extrachromosomal viral DNA. The genomes of papillomaviruses can also be stably maintained as high copy number extrachromosomal elements in cell lines (Law et al., 1981) and within these lines the viral genomes replicate in synchrony with cellular DNA. The viral genome copy number remains constant overall but the genomes are replicated by a random choice mechanism (Gilbert and Cohen, 1987; Ravnan et al., 1992). Long term, stable maintenance of papillomavirus-derived plasmids requires expression of the E1 and E2 proteins, the replication origin and a region from the LCR, that has been designated a minichromosome maintenance element (MME) (Piirsoo et al., 1996). This element contains multiple high affinity E2 binding sites. Recent studies have shown that both the BPV1 E2 transactivator protein and BPV genomes are associated with cellular chromosomes at mitosis (Skiadopoulos and McBride, 1998; Lehman and Botchan, 1998). This could be the mechanism by which approximately equal numbers of viral genomes are segregated to daughter cells at cell division to ensure that all basal cells of a papilloma contain viral DNA .
The third stage of viral replication is vegetative DNA replication, which is required to generate progeny virus. Vegetative DNA replication only occurs as the basal cells of a papilloma migrate upwards and differentiate in the stratum spinosum layer. Increased expression of the E2 proteins also occurs in the stratum spinosum and may be important for amplification of viral DNA (Burnett et al., 1990). The E2 protein is important for initiation of viral DNA replication but it has also been shown that HPV-31 E2 can arrest cells in S phase (Frattini et al., 1997). Clearly this could be important for vegetative replication by allowing sustained synthesis of viral DNA. There appears to be a switch from bidirectional theta replication in the maintenance stage of replication to a rolling circle mode in the vegetative stage (Flores and Lambert, 1997). Little else is known about vegetative viral DNA replication because of the requirement for terminally differentiating keratinocytes and difficulties in reproducing these conditions in a culture system. However, great advances are being made by replicating papillomaviruses in organotypic raft cultures and in xenografts of mice and these systems are proving to be very useful in studying the entire viral life cycle (reviewed in Meyers and Laimins, 1994).
Papillomavirus transcription is regulated primarily by the viral E2 gene products. These proteins regulate transcription by binding to specific DNA sites located in the viral genomes (see Figure 2). In bovine papillomavirus type 1 several gene products are expressed from the E2 ORF and they have been shown to function as transcriptional activators and repressors (Figure 3). cDNA species that could potentially encode truncated human papillomavirus E2 repressor proteins have been cloned but, as yet, no such proteins have been identified. Some HPVs may have evolved a mechanism to both activate and repress viral transcription with the full-length E2 protein (see Figure 5, reviewed in McBride and Myers, 1997; Fuchs and Pfister, 1994).
The full-length E2 protein from all papillomaviruses consists of a 200 amino acid N-terminal transactivation domain linked to a 100 amino acid C-terminal DNA binding and dimerization domain by a flexible hinge region of variable length and sequence (reviewed in McBride and Myers, 1997; McBride et al., 1989; Dostatni et al., 1988). The E2-TA protein activates transcription by binding to specific DNA binding sites that are located within enhancer elements in the viral genome (reviewed in McBride and Myers, 1997). There are seventeen different E2 binding sites in the BPV-1 genome that vary in affinity for the E2 protein over two orders of magnitude (Li et al., 1989) (Figure 2). The well-studied genital-associated HPV genomes contain only four E2 sites in the LCR (Figure 5).
The C-terminal domain of E2 binds specifically to DNA as a dimer. The X-ray crystal structure of the C-terminal 85 amino acids of E2 bound to DNA was the first example of an anti-parallel b-barrel DNA binding structure (Hegde et al., 1992). The DNA binding domain forms an eight-stranded anti-parallel b-barrel made up of four strands from each subunit. A pair of a-helices symmetrically positioned on the outside of the barrel contain the amino acids residues that are required for specific DNA interaction. The DNA binding domain of the Epstein Barr virus EBNA1 protein has a very similar structure to the E2 DNA binding domain despite no sequence similarity (Bochkarev et al., 1995).
The 200 amino acid E2 transactivation domain, unlike many other transactivation domains, appears to have a very constrained structure that is easily disrupted by deletion or certain non-conservative point mutations (reviewed in McBride and Myers, 1997). The transactivation domain is also critical for the replication function of the E2 protein and for interaction with the E1 protein (reviewed in McBride and Myers, 1997). The exact mechanism of transactivation has not been elucidated but probably involves interaction with components of the basic transcriptional machinery. BPV1 E2 has been shown to interact with SP1, TBP, TFIIB and a novel cellular protein, AMF-1 (Li et al., 1991; Steger et al., 1995; Rank and Lambert, 1995; Breiding et al., 1997).
In BPV-1, the E2 ORF encodes three different polypeptides; the E2-TA transactivator protein is encoded by the entire ORF and two smaller polypeptides, E2-TR and E8/E2, are encoded by the 3' half of the ORF. The shorter polypetides function as transcriptional repressors by antagonizing the function of E2-TA (Hubbert et al., 1988; Lambert et al., 1987; Spalholz et al., 1985; Lambert et al., 1989; Choe et al., 1989). The repressors contain only the DNA binding/dimerization domain and function both by direct competition with E2-TA for binding to the E2 DNA binding sites and by the formation of inactive heterodimers with the full-length E2-TA protein (Lim et al., 1998; Barsoum et al., 1992) (Figure 5).
In several HPVs associated with the anogenital tract, the full length E2 protein appears to repress the promoter located upstream from the E6 gene (reviewed in McBride and Myers, 1996, 1997; Fuchs and Pfister, 1994). This probably occurs when the E2 dimer binds to E2 DNA binding sites that overlap binding sites for the cellular SP1 and TFIID transcription factors. Recent studies have indicated that these proximal E2 binding sites have lower affinity for the E2 protein than the E2 binding sites are located further upstream from the promoter start site. This has led to a model in which low levels of E2 bind to the higher affinity upstream E2 sites and activate transcription, but at high levels of E2 protein the lower affinity proximal E2 sites are occupied leading to transcriptional repression (Figure 5). The situation is probably even more complex in a papilloma as the levels and activities of the E2 proteins and cellular transcription factors are likely modulated by cell cycle and epithelial differentiation.
Figure 5. Mechanisms of transcriptional regulation by the papillomavirus E2 proteins. A. BPV1 expresses a transcriptional transactivator with a transactivation domain and DNA binding/dimerization domain. Two shorter repressor proteins contain only the DNA binding/dimerization domain and repress E2 transactivation by forming heterodimers with the transactivator and by competing for the binding to the E2 sites in the viral genome. B. In many HPVs the full-length E2 protein can activate transcription by interacting with higher affinity E2 binding sites upstream from the transcriptional start site. At higher levels of E2, the lower affinity sites close to the promoter become occupied. This displaces essential cellular factors, SP1 and TFIID and results in repression of basal promoter activity.
Figure 6. Model of initiation of viral DNA replication. The E1 and E2 proteins initiate DNA replication by cooperatively binding to specific sites in the viral origin of replication. It has been proposed that an E1-alone complex then assembles in a ring-like hexamer structure around the DNA and the helicase activity of E1 unwinds the origin to allow access of the cellular replication machinery.
The viral E1 replication protein can also function as a transcriptional repressor. Inactivation of E1 increases the immortalizing or growth transforming potential of HPV-16 and BPV-1, respectively (Schiller et al., 1989; Lambert and Howley, 1988; Romanczuk and Howley, 1992) and this correlates well with the frequent disruption of E1 and/or E2 expression found in HPV-associated carcinomas. The E1 protein of BPV-1 can repress E2-mediated transactivation of the viral P89 promoter, which expresses the E6 and E7 gene products (Sandler et al., 1993; Le Moal et al., 1994). This is probably a consequence of binding of an E1/E2 complex to the replication origin, which is located just upstream from P89.
In addition to the cell’s replication machinery, papillomavirus DNA replication requires the full-length E2 transactivator protein, the viral E1 protein and the replication origin (Ustav and Stenlund, 1991a; Ustav et al., 1991b; Ustav and Stenlund, 1991a). The minimal origin of replication consists of an E1 binding site, an E2 binding site and an AT rich region that may facilitate origin unwinding. (Ustav et al., 1991b). The E1 protein has several replication-associated activities such as origin-specific binding (Wilson and Ludes-Meyers, 1991) and helicase activities (Yang et al., 1993) and forms a complex with the E2 transactivator (Mohr et al., 1990; Blitz and Laimins, 1991; Seo et al., 1993; Spalholz et al., 1993; Sedman and Stenlund, 1995) (Figure 4). The E1 and E2 sites have relatively low affinity for their respective proteins but together they cooperatively bind to the origin with high affinity (Figure 6). After the initial binding of an E1/E2 complex to the origin, the E1 protein oligomerizes to form a trimer or hexamer that encircles the DNA and E2 dissociates from the origin (Sedman and Stenlund, 1996, 1998). The E1 helicase function of the hexamer then unwinds the DNA at the origin to allow DNA synthesis to begin (Sedman and Stenlund, 1998) (Figure 6).
The E2-TA transactivator plays an auxiliary role in replication by enhancing and regulating the functions of the E1 protein. In addition to cooperatively binding to the origin with the E1 protein, E2 alleviates repression of replication by nucleosomes (Li and Botchan, 1994) and interacts with cellular replication proteins such as RPA (Li and Botchan, 1993).
Rodent cells transformed by BPV-1 maintain approximately 50 to 200 copies of the viral genome indefinitely as extrachromosomal nuclear plasmids (Law et al., 1981). Cell lines derived from cervical carcinomas can also maintain human papillomavirus genomes as extrachromosomal elements (Bedell et al., 1991). Plasmids containing the minimal viral replication origin replicate transiently in cells expressing the E1 and E2 proteins but the replicated DNA is lost with time. Long-term stable maintenance of origin-containing plasmids also requires regions from the LCR that contain multiple high affinity E2 DNA binding sites in addition to the replication origin (Piirsoo et al., 1996). This region has been designated the minichromosome maintenance element (MME) and can be substituted by ten tandem copies of E2 DNA binding sites, suggesting that the E2 protein and the E2 DNA binding sites are important for genome segregation (Figure 7). This finding is supported by the observation that the E2-TA protein and BPV-1 genomes are associated with
condensed mitotic chromosomes in dividing cells (Skiadopoulos and McBride, 1998; Lehman and Botchan, 1998) (Figure 8) and supports a model in which viral genomes are attached to mitotic chromatin indirectly via the E2 protein and E2 DNA binding sites (Figure 9). This interaction would ensure that approximately equal numbers of viral genomes are segregated to daughter cells. Viral genomes that replicate as extrachromosomal plasmids may also require a mechanism to ensure that they are not lost from the nucleus during cell division. Association with cellular chromosomes would ensure that viral genomes are enclosed in the nuclear membrane during telophase. The genomes may also interact with some cellular component that ensures that they are in a transcriptionally active region of the nucleus as the cells move into the G1 stage of the cell cycle.
The BPV-1 E2-TA protein interacts with mitotic chromatin in the absence of viral genomes. Conversely, the E2-TR and E8/E2 proteins are dispersed throughout the cell during mitosis and are excluded from mitotic chromatin. This indicates that the DNA binding domain of the E2 protein is not sufficient for the interaction with mitotic chromosomes and suggests that the interaction is not mediated by binding to cellular DNA sequences. The finding that a DNA-binding defective E2-TA protein retains the ability to interact with mitotic chromatin also supports this. Furthermore, deletions within the N-terminal domain abrogate the ability of E2 to interact with mitotic chromosomes. These findings indicate that the N-terminal transactivation domain of E2-TA is necessary for the interaction (Skiadopoulos and McBride, 1998).
As yet, it is not known what component of mitotic chromatin is important for interaction of the E2 protein with mitotic chromatin. One possibility is that E2 interacts with some constituent of the chromosomal scaffold or chromosomal periphery. The chromosomal periphery is a region around the condensed chromatids that contains many proteins, some of which form a network of fibrils and granules (Hernandez-Verdun and Gautier, 1994). Several components of the nuclear matrix are found in the perichromosomal region as well as a number of “passenger” proteins from the nucleus and nucleoli. The E2-TA protein (but not the E2-TR or E8/E2 proteins) has been shown to be associated with the nuclear matrix (Hubbert et al., 1988) and it will be interesting to determine whether the same interactions are important for the association with mitotic chromosomes. Nuclear matrix attachment sites have also been identified in the BPV-1 genome (Adom and Richard-Foy, 1991; Adom et al., 1992; Tan et al., 1998) and it is possible that these sites are also important for interaction of the genomes with mitotic chromatin instead of, or in addition to, E2 DNA binding sites.
Although the overall viral copy number in a population of BPV-1 transformed cells remains relatively constant, several studies have shown that individual cells contain a wide range of copy numbers (Roberts and Weintraub, 1988; Ravnan et al., 1992; Ravnan and Cohen, 1997). Stewart et al. (1994) also demonstrated that there is significant randomization in replication and/or partitioning. This suggests that segregation does not occur by a very precise mechanism and is consistent with the model that the E2 proteins and viral genomes randomly associate with mitotic chromatin as passenger molecules. This model would also predict that the viral copy number depends on the levels of the E2-TA protein.
A similar phenomenon has been observed for Epstein-Barr virus (EBV). EBV infects and immortalizes B-lymphocytes and the viral genome is maintained indefinitely as an extrachromosomal element. The EBNA-1
Figure 8. Papillomavirus genomes and the E2-TA transactivator protein are associated with cellular chromosomes in mitotic cells. E2 proteins were detected in COS7 cells expressing the E2-TA protein by indirect immunofluorescence using an E2-specific antibody. Panels A and B show COS-7 cells as a control. Panels C and D show COS-7 cells expressing E2-TA. In panels A and C, cellular DNA was detected by propidium iodide staining. In panels B and D, FITC-labeled E2 protein is detected in the same field of cells. BPV DNA was detected by fluorescent in situ hybridization in C127 cells (E and F) and 137 cells (that contain BPV-1) (G and H). In panels E and G cellular DNA was detected by the propidium iodide signal. In panels F and H the same field of cells are shown with the FITC-labeled BPV DNA signal. Mitotic cells are indicated by white arrows.
Figure 9. This diagram shows a model in which papillomavirus genomes are linked via the E2-TA protein to condensed mitotic chromosomes.
protein of EBV is a transcriptional transactivator and a replication protein and it is the only viral protein required for replication and maintenance of plasmids containing the oriP origin of replication (which contains a number of repeated EBNA DNA binding sites) (Yates et al., 1985). The EBNA 1 protein and EBV genomes have also been shown to be randomly associated with mitotic chromatin (Grogan et al., 1983; Harris et al., 1985) and it has been suggested that these properties might be important for the genome segregation and nuclear retention function of EBNA-1. The EBNA-1 protein also promotes prolonged nuclear retention of plasmids containing EBNA-1 DNA binding sites but no origin of replication (Krysan et al., 1989; Middleton and Sugden, 1994) and it has been proposed that this is due to the interaction of plasmids with mitotic chromosomes.
The Epstein-Barr virus EBNA-1 protein and the papillomavirus E2-TA protein have common roles in the life cycles of their respective viruses (Grossman and Laimins, 1996). Both proteins are transcriptional transactivators that activate transcription by binding to specific binding sites within the viral genomes. Notably, both proteins have dimeric DNA binding. domains with almost identical anti-parallel b-barrel structures, despite no amino acid homology (Bochkarev et al., 1995). Both viruses replicate and maintain their genomes as extrachromosomal elements in persistently infected cells. This maintenance requires both E2-TA and the multiple E2 binding sites in the MME element of papillomavirus (Piirsoo et al., 1996) or EBNA-1 and the multiple EBNA-1 binding sites in the oriP element of Epstein-Barr virus (Yates et al., 1985). In both cases the viral proteins and genomes are associated with condensed cellular chromosomes during mitosis (Skiadopoulos and McBride, 1998; Lehman and Botchan, 1998; Grogan et al., 1983, Harris et al., 1985). The EBNA-1 protein promotes prolonged nuclear retention of plasmids containing EBNA-1 DNA binding sites even in the absence of replication (Krysan et al., 1989; Middleton and Sugden, 1994) and it has been proposed that this is due to the association with mitotic chromosomes.
Gene therapy vectors that replicate and are retained extrachromosomally have several advantages over those that integrate in a random fashion into the host genome. These vectors will persist in proliferating cells and should not generate mutations by insertion into the cellular chromosomes. Such vectors can be maintained at a high copy number and are not susceptible to positional effects, such as inactivation, that are dependent on the integration site. EBV-based vectors that contain the EBNA-1 gene and the oriP replication origin have been developed and can express foreign gene products in primate and human cells (reviewed in Calos, 1996). Another class of EBV vectors have been developed that only contain the EBNA-1 gene and repeats of the EBNA-1 binding site required for nuclear retention. In these vectors the oriP origin has been replaced with a cellular replication origin and the resulting vectors are able to replicate in a wider range of mammalian cells (Krysan et al., 1989). The EBNA-1 protein also has the advantage that it is not recognized by the cell-mediated immune system (Levitskaya et al., 1995) as it is resistant to the proteasome-mediated degradation that is required for antigen presentation (Levitskaya et al., 1997). However, there is a report that the EBNA-1 protein can cause lymphomas in transgenic mice expressing this protein in B-cells (Wilson et al., 1996).
Sarver et al. (1981) first described the use of papillomaviruses as vectors in 1981. In general, these vectors comprised the 69% transforming region of the virus (the genome minus the late region) and the foreign gene to be expressed. A newer vector only contains the LCR and the E1 and E2 genes (Ohe et al., 1995). However, these vectors have a limited host range and, in some cases, insertion of a foreign transcriptionally active foreign gene causes the plasmid to integrate (Waldenstrom et al., 1992). This is probably because the small papillomavirus genomes are very compact and contain multiple overlapping genes and regulatory signals that can be inadvertently disrupted. The presence of an active heterologous enhancer and promoter could interfere with viral replication by transcriptional interference. Using the detailed knowledge of the mechanisms of papillomavirus replication and genome maintenance, it should be possible to generate a new class of papillomavirus vectors. These vectors could express the E1 and/or E2 genes from different promoters suitable for a specific cell type and either viral or cellular replication origins could be incorporated, as has been described for EBV-based vectors (Krysan et al., 1989). The addition of repeated E2 binding sites may be sufficient to maintain the vector as an episome when either a viral or cellular replication origin is used.
Adom, J.N., Gouilleux, F., and Richard-Foy, H. (1992). Interaction with the nuclear matrix of a chimeric construct containing a replication origin and a transcription unit. Biochim. Biophys. Acta 1171, 187-197.
Adom, J.N. and Richard-Foy, H. (1991). A region immediately adjacent to the origin of replication of bovine papilloma virus type 1 interacts in vitro with the nuclear matrix. Biochem. Biophys. Res. Commun. 176, 479-485.
Barsoum, J., Prakash, S.S., Han, P., and Androphy, E.J. (1992). Mechanism of action of the papillomavirus E2 repressor: repression in the absence of DNA binding. J. Virol. 66, 3941-3945.
Bedell, M.A., Hudson, J.B., Golub, T.R., Turyk, M.E., Hosken, M., Wilbanks, G.D., and Laimins, L.A. (1991). Amplification of human papillomavirus genomes in vitro is dependent on epithelial differentiation. J. Virol. 65, 2254-2260.
Blitz, I.L. and Laimins, L.A. (1991). The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro. J. Virol. 65, 649-656.
Bochkarev, A., Barwell, J.A., Pfuetzner, R.A., Furey, W.J., Edwards, A.M., and Frappier, L. (1995). Crystal structure of the DNA-binding domain of the Epstein-Barr virus origin-binding protein EBNA 1. Cell 83, 39-46.
Breiding, D., Sverdrup, F., Grossel, M.J., Moscufo, N., Boonchai, W., and Androphy, E.J. (1997). Isolation of a BPV1 E2 transactivation domain binding factor required for both transcriptional activation and DNA replication. Virology 221, 34-43.
Burnett, S., Strom, A.C., Jareborg, N., Alderborn, A., Dillner, J., Moreno-Lopez, J, Pettersson, U., and Kiessling, U. (1990). Induction of bovine papillomavirus E2 gene expression and early region transcription by cell growth arrest: correlation with viral DNA amplification and evidence for differential promoter induction. J. Virol. 64, 5529-5541.
Calos, M.P. (1996). The potential of extrachromosomal replicating vectors for gene therapy. Trends. Genet. 12, 463-466.
Choe, J., Vaillancourt, P., Stenlund, A., and Botchan, M. (1989). Bovine papillomavirus type 1 encodes two forms of a transcriptional repressor: Structural and functional analysis of new viral cDNAs. J. Virol. 63, 1743-1755.
Dostatni, N., Thierry, F., and Yaniv, M. (1988). A dimer of BPV-1 E2 containing a protease resistant core interacts with its DNA target. EMBO J. 7, 3807-3816.
Evander, M., Frazer, I.H., Payne, E., Qi, Y.M., Hengst, K., and McMillan, N.A. (1997). Identification of the a6 integrin as a candidate receptor for papillomaviruses. J. Virol. 71, 2449-2456.
Flores, E.R. and Lambert, P.F. (1997). Evidence for a switch in the mode of human papillomavirus type 16 DNA replication during the viral life cycle. J. Virol. 71, 7167-7179.
Frattini, M.G., Hurst, S.D., Lim, H.B., Swaminathan, S., and Laimins, L.A. (1997). Abrogation of a mitotic checkpoint by E2 proteins from oncogenic human papillomaviruses correlates with increased turnover of the p53 tumor suppressor protein. EMBO J. 16, 318-331.
Fuchs, P.G. and Pfister, H. (1994). Transcription of papillomavirus genomes. Intervirology 37, 159-167.
Gilbert, D.M. and Cohen, S.N. (1987). Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle. Cell 50, 59-68.
Grogan, E.A., Summers, W.P., Dowling, S., Shedd, D., Gradoville, L., and Miller, G. (1983). Two Epstein-Barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding. Proc. Natl. Acad. Sci. USA 80, 7650-7653.
Grossman, S.R. and Laimins, L.A. (1996). EBNA1 and E2: a new paradigm for origin-binding proteins? Trends. Microbiol. 4, 87-89.
Harris, A., Young, B.D., and Griffin, B.E. (1985). Random association of Epstein-Barr virus genomes with host cell metaphase chromosomes in Burkitt's lymphoma-derived cell lines. J. Virol. 56, 328-332.
Hegde, R.S., Grossman, S.R., Laimins, L.A., and Sigler, P.B. (1992). Crystal structure at 1.7 A of the bovine papillomavirus-1 E2 DNA- binding domain bound to its DNA target. Nature 359, 505-512.
Hernandez-Verdun, D. and Gautier, T. (1994). The chromosome periphery during mitosis. Bioessays 16, 179-185.
Howley, P.M.(1995). Papillomavirinae: The viruses and their replication. In Virology (Fields, B.N., Knipe, D.M., and Howley, P.M., Eds.) Lippincott-Raven, Philadelphia and New York. 2045-2076.
Hubbert, N.L., Schiller, J.T., Lowy, D.R., and Androphy, E.J. (1988). Bovine papilloma virus-transformed cells contain multiple E2 proteins. Proc. Natl. Acad. Sci. USA 85, 5864-5868.
Jones, D.L. and Munger, K. (1996). Interactions of the human papillomavirus E7 protein with cell cycle regulators. Semin. Cancer Biol. 7, 327-337.
Krysan, P.J., Haase, S.B., and Calos, M.P. (1989). Isolation of human sequences that replicate autonomously in human cells. Mol. Cell Biol. 9, 1026-1033.
Kubbutat, M.H.G. and Vousden, K.H. (1996). Role of E6 and E7 oncoproteins in HPV-induced anogenital malignancies. Semin.Virol. 7, 295-304.
Lambert, P.F. and Howley, P.M. (1988). Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation. J. Virol. 62, 4009-4015.
Lambert, P.F., Hubbert, N.L., Howley, P.M., and Schiller, J.T. (1989). Genetic assignment of multiple E2 gene products in bovine papillomavirus-transformed cells. J. Virol. 63, 3151-3154.
Lambert, P.F., Spalholz, B.A., and Howley, P.M. (1987). A transcriptional repressor encoded by BPV-1 shares a common carboxy-terminal domain with the E2 transactivator. Cell 50, 69-78.
Law, M.F., Lowy, D.R., Dvoretzky, I., and Howley, P.M. (1981). Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc. Natl. Acad. Sci. USA 78, 2727-2731.
Le Moal, M.A., Yaniv, M., and Thierry, F. (1994). The bovine papillomavirus type 1 (BPV1) replication protein E1 modulates transcriptional activation by interacting with BPV1 E2. J. Virol. 68, 1085-1093.
Lehman, C.W. and Botchan, M.R. (1998). Segregation of viral plasmids depends on tethering to chromosomes and is regulated by phosphorylation. Proc. Natl. Acad. Sci. USA 95, 4338-4343.
Levitskaya, J., Coram, M., Levitsky, V., Imreh, S., Steigerwald-Mullen, P.M., Klein, G., Kurilla, M.G., and Masucci, M.G. (1995). Inhibition of antigen processing by the internal repeat region of the Epstein-Barr virus nuclear antigen-1. Nature 375, 685-688.
Levitskaya, J., Sharipo, A., Leonchiks, A., Ciechanover, A., and Masucci, M.G. (1997). Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat domain of the Epstein-Barr virus nuclear antigen 1. Proc. Natl. Acad. Sci. USA 94, 12616-12621.
Li, R. and Botchan, M.R. (1993). The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication. Cell 73, 1207-1221.
Li, R. and Botchan, M.R. (1994). Acidic transcription factors alleviate nucleosome-mediated repression of DNA replication of bovine papillomavirus type 1. Proc. Natl. Acad. Sci. USA 91, 7051-7055.
Li, R., Knight, J., Bream, G., Stenlund, A., and Botchan, M. (1989). Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV- 1 genome. Genes Dev. 3, 510-526.
Li, R., Knight, J.D., Jackson, S.P., Tjian, R., and Botchan, M.R. (1991). Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell 65, 493-505.
Lim, D.A., Gossen, M., Lehman, C.W., and Botchan, M.R. (1998). Competition for DNA binding sites between the short and long forms of E2 dimers underlies repression in bovine papillomavirus type 1 DNA replication control. J. Virol. 72, 1931-1940.
McBride, A.A., Byrne, J.C., and Howley, P.M. (1989). E2 polypeptides encoded by bovine papillomavirus type 1 form dimers through the common carboxyl-terminal domain: Transactivation is mediated by the conserved amino-terminal domain. Proc. Natl. Acad. Sci. USA 86, 510-514.
McBride, A.A. and Myers, G.(1996). The E2 proteins .in Human Papillomaviruses (Myers, G. , Baker, C., Wheeler, C., Halpern, A., McBride, A., and Doorbar, J., Eds.) .Los Alamos National Laboratory, Los Alamos.
McBride, A.A. and Myers, G.(1997). The E2 proteins: an update .in Human Papillomaviruses 1997 (Myers, G., Baker, C., Munger, K., Sverdrup, F., McBride, A. , and Bernard, H.-U., Eds.) .Los Alamos National Laboratory, Los Alamos. http://hpvweb.lanl.gov/
Meyers, C. and Laimins, L.A. (1994). In vitro systems for the study and propagation of human papillomaviruses. Curr. Top. Microbiol. Immunol. 186, 199-215.
Middleton, T. and Sugden, B. (1994). Retention of plasmid DNA in mammalian cells is enhanced by binding of the Epstein-Barr virus replication protein EBNA1. J. Virol. 68, 4067-4071.
Mohr, I.J., Clark, R., Sun, S., Androphy, E.J., MacPherson, P., and Botchan, M.R. (1990). Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science 250, 1694-1699.
Ohe, Y., Zhao, D., Saijo, N., and Podack, E.R. (1995). Construction of a novel bovine papillomavirus vector without detectable transforming activity suitable for gene transfer. Hum. Gene Ther. 6, 325-333.
Piirsoo, M., Ustav, E., Mandel, T., Stenlund, A., and Ustav, M. (1996). Cis and trans requirements for stable episomal maintenance of the BPV-1 replicator. EMBO J. 15, 1-11.
Rank, N.M. and Lambert, P.F. (1995). Bovine papillomavirus type 1 E2 transcriptional regulators directly bind two cellular transcription factors, TFIID and TFIIB. J. Virol. 69, 6323-6334.
Ravnan, J.-B. and Cohen, S.N. (1997). Transformed mouse cell lines that consist predominantly of cells maintaining bovine papillomavirus at high copy number. Virology 213, 526-534.
Ravnan, J.-B., Gilbert, G.M., Ten Hagen, K.G., and Cohen, S.N. (1992). Random-choice replication of extrachromosomal bovine papillomavirus (BPV) molecules in heterogeneous clonally-derived BPV-infected cell lines. J. Virol. 66, 6946-6952.
Roberts, J.M. and Weintraub, H. (1988). Cis-acting negative control of DNA replication in eukaryotic cells. Cell 52, 397-404.
Romanczuk, H. and Howley, P.M. (1992). Disruption of either the E1 or the E2 regulatory gene of human papillomavirus type 16 increases viral immortalization capacity. Proc. Natl. Acad. Sci. USA 89, 3159-3163.
Sandler, A.B., Vande Pol, S.B., and Spalholz, B.A. (1993). Repression of bovine papillomavirus type 1 transcription by the E1 replication protein. J. Virol. 67, 5079-5087.
Sarver, N., Gruss, P., Law, M.F., Khoury, G., and Howley, P.M. (1981). Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol.Cell.Biol. 1, 486-496.
Schiller, J.T., Kleiner, E., Androphy, E.J., Lowy, D.R., and Pfister, H. (1989). Identification of bovine papillomavirus E1 mutants with increased transforming and transcriptional activity. J. Virol. 63, 1775-1782.
Sedman, J. and Stenlund, A. (1995). Co-operative interaction between the initiator E1 and the transcriptional activator E2 is required for replicator specific DNA replication of bovine papillomavirus in vivo and in vitro. EMBO J. 14, 6218-6228.
Sedman, J. and Stenlund, A. (1996). The initiator protein E1 binds to the bovine papillomavirus origin of replication as a trimeric ring-like structure. EMBO J. 15, 5085-5092.
Sedman, J. and Stenlund, A. (1998). The papillomavirus E1 protein forms a DNA-dependent hexameric complex with ATPase and DNA helicase activities. J. Virol. 72, 6893-6897.
Seo, Y.-S., Muller, F., Lusky, M., Gibbs, E., Kim, H.-Y., Phillips, B., and Hurwitz, J. (1993). Bovine papilloma virus (BPV)-encoded E2 protein enhances binding of E1 protein to the BPV replication origin. Proc. Natl. Acad. Sci. USA 90, 2865-2869.
Skiadopoulos, M.H. and McBride, A.A. (1998). BPV1 viral genomes and the E2 transactivator protein are associated with cellular metaphase chromosomes. J Virol 72, 2079-2088.
Spalholz, B.A., McBride, A.A., Sarafi, T., and Quintero, J. (1993). Binding of bovine papillomavirus E1 to the origin is not sufficient for DNA replication. Virology 193, 201-212.
Spalholz, B.A., Yang, Y.C., and Howley, P.M. (1985). Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product. Cell 42, 183-191.
Steger, G., Ham, J., Lefebvre, O., and Yaniv, M. (1995). The bovine papillomavirus 1 E2 protein contains two activation domains: one that interacts with TBP and another that functions after TBP binding. EMBO J. 14, 329-340.
Stewart, A.-C., Jareborg, N., Simonsson, M., Alderborn, A., and Burnett, S. (1994). Segregation properties of bovine papillomaviral plasmid DNA. J.Mol.Biol. 236, 480-490.
Tan, S.H., Bartsch, D., Schwarz, E., and Bernard, H.U. (1998). Nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses. J. Virol. 72, 3610-3622.
Ustav, M. and Stenlund, A. (1991a). Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames. EMBO J. 10, 449-457.
Ustav, M., Ustav, E., Szymanski, P., and Stenlund, A. (1991b). Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1. EMBO J. 10, 4321-4329.
Waldenstrom, M., Schenstrom, K., Sollerbrant, K., and Hansson, L. (1992). Replication of bovine papillomavirus vectors in murine cells. Gene 120, 175-181.
Wilson, J.B., Bell, J.L., and Levine, A.J. (1996). Expression of Epstein-Barr virus nuclear antigen-1 induces B cell neoplasia in transgenic mice. EMBO J. 15, 3117-3126.
Wilson, V.G. and Ludes-Meyers, J. (1991). A bovine papillomavirus E1-related protein binds specifically to bovine papillomavirus DNA. J. Virol. 65, 5314-5322.
Yang, L., Li, R., Mohr, I.J., Clark, R., and Botchan, M.R. (1991). Activation of BPV-1 replication in vitro by the transcription factor E2. Nature 353, 628-632.
Yang, L., Mohr, I., Fouts, E., Lim, D.A., Nohaile, M., and Botchan, M. (1993). The E1 protein of bovine papillomavirus 1 is an ATP-dependent DNA helicase. Proc. Natl. Acad. Sci. USA 90, 5086-5090.
Yates, J.L., Warren, N., and Sugden, B. (1985). Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature 313, 812-815.
Zhou, J., Gissmann, L., Zentgraf, H., Muller, H., Picken, M., and Muller, M. (1995). Early phase in the infection of cultured cells with papillomavirus virions. Virology 214, 167-176.